Feb 14, 2020 · The inf in x-axis means number of reads filtered due to too many alignments. For the pie chart, four categories of reads --- unalignable, unique, isoform-levelmulti-mapping, filtered -- are plotted and their percentages are noted. In both the histogram and the piechart, numbers belong to unalignable, unique, multi-mapping, and filtered are ...

It uses junction reads to estimate exon exclusion and reads mapped within exon to estimate its inclusion. SAJR models it by GLM with quasibinomial distribution and uses log likelihood test to assess significance. Scotty Performs power analysis to estimate the number of replicates and depth of sequencing required to call differential expression.

Tips Check what factors regulate your gene of interest, what factors bind in your interval or have a significant binding overlap with your peak set.

Nov 27, 2020 · The number of reads mapped to either the first 21,500 nucleotides (nt) ... Checking of mapped reads indicated that they were all mapped uniquely and thus counted only once.

总碱基数量、Totally mapped reads、Uniquely mapped reads统计，测序深度分析。 2．一致性序列组装 与参考基因组序列（Reference genome sequence）的比对分析，利用 贝叶斯统计 模型检测出每个碱基位点的最大可能性基因型，并组装出该 个体基因组 的 一致序列 。