chromatography wikipedia - EAS
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In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the … See more
Chromatography, pronounced /ˌkroʊməˈtɒɡrəfi/, is derived from Greek χρῶμα chroma, which means "color", and γράφειν graphein, which means "to write". The combination of these two terms was directly inherited … See more
Chromatography was first devised in Russia by the Italian-born scientist Mikhail Tsvet in 1900. He developed the technique and coined the term … See more
Column chromatography
Column chromatography is a separation technique in which the stationary bed is within a tube. The particles of the solid stationary phase or the support coated with a liquid stationary phase may fill the whole inside … See moreGas chromatography
Gas chromatography (GC), also sometimes known as gas-liquid chromatography, (GLC), is a separation technique in which the mobile phase is a gas. Gas chromatographic separation is always carried out … See more• Analyte – the substance to be separated during chromatography. It is also normally what is needed from the mixture.
• Analytical chromatography – the use of chromatography to … See moreThe basic principle of displacement chromatography is: A molecule with a high affinity for the chromatography matrix (the displacer) competes … See more
Affinity chromatography is based on selective non-covalent interaction between an analyte and specific molecules. It is very specific, but not very robust. It is often used in biochemistry in the purification of proteins bound to tags. These fusion proteins are … See more
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