WebMassive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing.Some of these technologies emerged between 1994 and 1998 and have …

WebSanger sequencing is a method of DNA sequencing that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.After first being developed by Frederick Sanger and colleagues in 1977, it became the most widely used sequencing method for …

WebParts of this article (those related to long-read sequencing technologies producing low-accuracy reads. While true 5 years ago, circular consensus reads with the PacBio Sequel II long-read sequencer can easily achieve an even higher read accuracy than hybrid genome assembly with a combination of other sequencers. PMID 31885515, 28364362, …

WebDNA sequencing is the process of determining the nucleotide order of a given DNA fragment. So far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger.This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates.

WebIllumina dye sequencing is a technique used to determine the series of base pairs in DNA, also known as DNA sequencing. The reversible terminated chemistry concept was invented by Bruno Canard and Simon Sarfati at the Pasteur Institute in Paris. It was ...

WebChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA.ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest. Previously, …

WebSequencing batch reactors (SBR) or sequential batch reactors are a type of activated sludge process for the treatment of wastewater.SBR reactors treat wastewater such as sewage or output from anaerobic digesters or mechanical biological treatment facilities in batches. Oxygen is bubbled through the mixture of wastewater and activated sludge to …

WebThe Human Genome Project (HGP) was an international scientific research project with the goal of determining the base pairs that make up human DNA, and of identifying, mapping and sequencing all of the genes of the human genome from both a physical and a functional standpoint. It started in 1990 and was completed in 2003. It remains the world's …

WebProtein sequencing is the practical process of determining the amino acid sequence of all or part of a protein or peptide.This may serve to identify the protein or characterize its post-translational modifications.Typically, partial sequencing of a protein provides sufficient information (one or more sequence tags) to identify it with reference to databases of …

WebThiruvananthapuram (/ ˌ t ɪ r uː v ə ˌ n ʌ n t ə ˈ p ʊər ə m /; Malayalam: [t̪iɾuʋɐnɐn̪d̪ɐpuɾɐm] ()), also known by its former name Trivandrum (/ t r ɪ ˈ v æ n d r ə m /), is the capital of the Indian state of Kerala.It is the most populous city in Kerala with a population of 957,730 as of 2011. The encompassing urban agglomeration population is around 1.68 million.